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Instructions for Using ANAC Reagent
Storage: ANAC powder is recommended to store at < -20°C.
Use:
1,
Dissolve ANAC reagent to Milli-Q®️ water or a buffer recommended at least one hour before use.
(ANAC solution can be stored at 4°C)
2,
Add ANAC solution to your reaction. Recommended weight ratio of ANAC reagent to DNA (or RNA) is 8 in the reaction. The weight ratio would be refined from 2 to 100.
Recommended buffer: Phosphate buffered saline (PBS), 10 mM HEPES + 140 mM NaCl (or KCl)
Concentration of divalent cations should be < 10 mM.
Caution:
SDS and other ionic surfactants, dextran sulfate and other ionic polymers could inhibit activity of ANAC reagent.
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